高铜日粮对肉鸡肾氧化损伤和Nrf2信号通路相关基因表达的影响

doi:10.11843/j.issn.0366-6964.2019.09.020

摘要/Abstract

摘要： 为了研究高铜日粮对肉鸡肾氧化损伤和Nrf2信号通路相关基因表达的影响，选用48只1日龄健康白羽肉鸡，随机分为4组，每组12只，分别喂以基础日粮（Cu 11 mg·kg^-1，对照组）和高铜日粮（Cu 110 mg·kg^-1，高铜Ⅰ组；Cu 220 mg·kg^-1，高铜Ⅱ组；Cu 330 mg·kg^-1，高铜Ⅲ组），并于49日龄采集肾组织作为样品，制作组织切片观察肾病理变化，检测超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽还原酶（GR）和总抗氧化能力（T-AOC）等指标的变化，并通过实时荧光定量PCR检测核转录相关因子2（Nrf2）、谷氨酰半胱氨酸连接酶催化亚基（GCLC）、谷氨酰半胱氨酸合成酶修饰亚基（GCLM）、血红素氧化酶（HO-1）、NAD（P）H醌氧化还原酶1（NQO1）的mRNA相对转录量。结果显示，高铜组肉鸡的肾间质变宽，肾小管上皮细胞出现脱落；高铜Ⅲ组肾的T-AOC、SOD和GR活性与对照组相比显著下降；MDA含量无显著变化；高铜Ⅱ组和高铜Ⅲ组Nrf2和GCLC mRNA相对转录量与对照组相比显著升高，高铜组GCLM和HO-1 mRNA相对转录量与对照组相比显著上调，而NQO1 mRNA的相对转录量则无显著变化。结果表明，高铜日粮可诱导肉鸡肾发生氧化损伤和Nrf2信号通路相关基因的表达。

Abstract: In order to investigate the effect of high dietary copper on oxidative damage and expression of Nrf2 signaling pathway related genes in the kidneys of broilers, 48 one-day-old healthy white feather broilers were randomly divided into 4 groups with 12 each in this study. The birds were fed with basal diets (Cu 11 mg·kg^-1, the control group), and high-copper diets (Cu 110 mg·kg^-1, the high-copper group Ⅰ; Cu 220 mg·kg^-1, the high-copper group Ⅱ; Cu 330 mg·kg^-1, the high-copper group Ⅲ, respectively). On day 49, the kidneys were collected for histopathology examination. The activities of superoxide dismutase (SOD), glutathione reductase (GR), and total antioxidant capacity (T-AOC); and the content of malondialdehyde (MDA) were detected. Additionally, mRNA levels of nuclear factor E2-related factor 2 (Nrf2), glutamate cysteine ligase catalytic subunit (GCLC), glutamate cysteine ligase modifier subunit (GCLM), hemeoxygenase-1 (HO-1), and NAD (P) H quinone oxidoreducetase 1 (NQO1) were measured using real-time quantitative PCR (RT-qPCR). The results showed that widen renal interstitial and exfoliated renal tubular epithelial cells were observed in the high-copper groups. Additionally, the activities of SOD, GR, and T-AOC in the high-copper group Ⅲ were significantly decreased compared to those in the control group, but there was no significant change in the content of MDA. Moreover, mRNA levels of Nrf2 and GCLC in the high-copper group Ⅱ and the high-copper group Ⅲ were markedly higher than those in the control group, and mRNA levels of GCLM and HO-1 up-regulated with the increase of dietary copper, but NQO1 mRNA level did not significantly changed. The results suggest that high dietary copper can induce oxidative damage and the expression of Nrf2 signaling pathway related genes in the kidneys of broilers.

中图分类号:

S856.9

裴若男, 杨帆, 廖建昭, 马飞洋, 马欣艳, 林毓茵, 姚启发, 唐兆新, 梁昭平. 高铜日粮对肉鸡肾氧化损伤和Nrf2信号通路相关基因表达的影响[J]. 畜牧兽医学报, 2019, 50(9): 1912-1919.

PEI Ruonan, YANG Fan, LIAO Jianzhao, MA Feiyang, MA Xinyan, LIN Yuyin, YAO Qifa, TANG Zhaoxin, LIANG Zhaoping. The Effect of High Dietary Copper on Oxidative Damage and Expression of Nrf2 Signaling Pathway Related Genes in the Kidneys of Broilers[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(9): 1912-1919.

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